Plasmid
pUC57

Part:BBa_J70004

Designed by: Meagan Lizarazo   Group: Knight Lab   (2008-01-23)
Revision as of 17:51, 23 January 2008 by Meaganl (Talk | contribs)

BioBasic Cloning Plasmid pUC57

Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.

Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal PstI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1790
    Illegal SapI site found at 707


[edit]
Categories
//plasmidbackbone/synthesis
Parameters
chassis
copies
mcs
origin
resistanceA