Generator
Wintergree

Part:BBa_J45181

Designed by: Stephen Payne   Group: iGEM06_MIT   (2007-02-10)

Exponential phase dependent wintergreen odor generator

The exponential phase dependent wintergreen odor generator (BBa_J45181) catalyzes the conversion of the precursor salicylic acid to the odor methyl salicylate that has a wintergreen smell. The biosynthetic device is composed of two transcriptional devices: a exponential phase transcription source (BBa_J45994) and an odor enzyme generator (BBa_J45119). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

BBa J45181.png

Usage and Biology

  • Supplement cultures with 2 mM salicylic acid to produce wintergreen odor.
  • See BBa_J45004 for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1799
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1340


[edit]
Categories
//function/odor
s
Parameters
None