Generator
WOG

Part:BBa_J45120

Designed by: MIT IGEM 2006   Group: iGEM06_MIT   (2006-08-10)

Wintergreen odor generator

The wintergreen odor generator (BBa_J45120) catalyzes the conversion of the precursor salicylic acid to the odor methyl salicylate that has a wintergreen smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0040) and an odor enzyme generator (BBa_J45119). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

BBa J45120.png

Usage and Biology

  • Supplement cultures with 2 mM salicylic acid to produce wintergreen odor.
  • See BBa_J45004 for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 285


[edit]
Categories
//classic/generator/prct
//function/odor
s
Parameters
o_h
proteinBSMT1
rbs149
tagNone