Generator
WOG

Part:BBa_J45100

Designed by: Boyuan Zhu   Group: iGEM2006_MIT   (2006-08-09)

Wintergreen odor enzyme (BSMT1) generator

This device produces methyl salicylate in the presence of salicylic acid. Expression is regulated by R0040 (Tet repressor). Methyl salicylate smells strongly of mint (wintergreen).

J45100.jpg

Wintergreen Generating Device (WGD):

  • converts salicylic acid (SA) into methyl salicylate
  • production of methyl salicylate was verified both by scent and by gas chromatography: E. coli with no WGD did not produce methyl salicylate when SA was added to the medium, while E. coli with the WGD did produce methyl salicylate when SA was added to the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287


[edit]
Categories
//classic/generator/prct
//function/odor
Parameters
o_h
proteinBSMT1
rbs147
tagNone