Coding
JAMT

Part:BBa_J45003

Designed by: Stephen Payne   Group: iGEM2006_MIT   (2006-06-07)

SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor

BBa_J45003 encodes SAM jasmonic acid carboxyl methyltransferase derived from JMT from Arabidopsis thaliana. JMT catalyzes the conversion of jasmonic acid to methyl jasmonate. Methyl jasmonate has a jasmine smell.

Usage and Biology

  • 1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
  • Seo et al. were able to get the enzyme, purified from E. coli, to catalyze the jasmine-smelling reaction in vitro.
  • Km: 38.5 μM; Kcat: 25 s−1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 613


[edit]
Categories
//function/odor
s
Parameters
ec_num
kegg
proteinJAMT
swisspro