RBS

Part:BBa_J435362

Designed by: Open Bioeconomy   Group: FreeGenes   (2023-05-09)


BN1_BCD2_DsbA

Riboj is a ribozyme that reduces DNA context from expression. Additionally contains DsbA tag. Defined as a BN1 part.

FLANKED 5' fusion site: TACT, 3' fusion site: CCAT

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003391.html

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 564
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 564
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 564
    Illegal AgeI site found at 184
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None