Part:BBa_J3107

AraC-PC, pLac-Hin

AraC-PC (part BBa_I13458) should constitutively express the AraC protein (inhibitor of the pBad promoter). Expression of Hin invertase is under control of the pLac promoter. Therefore, Hin should be expressed in the presence of IPTG. This part was designed for two reasons:

1. To regulate DNA inversion (via controlled Hin invertase expression) on a second plasmid 2. To regulate the transcription of a pBad driven reporter (via the addition of arabinose) on the second plasmid

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961