Generator

Part:BBa_J3106

Designed by: Davidson and Missouri Western   Group: iGEM2006_Davidson   (2006-08-02)


pBad-HixC-TetB-RBSrev-HixC-TT-RE

This part contains the tetracycline resistance in the backwards orientation as well as a backwards RBS. These parts are flanked by HixC sites so when this part is placed downstream of the Hin or HinLVA cassettes the RBS and the tetracycline resistance will be in the correct orientation and the cell will express tetracyline resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1215
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1069
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 515
    Illegal NgoMIV site found at 675
    Illegal NgoMIV site found at 1043
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/hin
Parameters
None