Generator

Part:BBa_J3104

Designed by: Davidson and Missouri Western   Group: iGEM2006_Davidson   (2006-08-01)

pBad-RBS-HixC-TetF-HixC-TT-RE

This part contains the gene for tetracycline resistance flanked by HixC regions. This part will be used to test if the ribosome will be able to read through the Hix site.


Usage and Biology

Note that there is no PC+AraC so this is a constitutive promoter. However, with the RBS separated from the start codon, no Tetracycline resistance is produced.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 509
    Illegal NgoMIV site found at 877
    Illegal NgoMIV site found at 1037
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/hin
Parameters
None