Coding
Hin

Part:BBa_J31000

Designed by: Erin Zwack, Sabriya Rosemond   Group: iGEM2006_Davidson   (2006-06-05)

DNA-invertase Hin from Salmonella typhimurium

Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix sites. The actual part sequence should have an A at base pair 70 and a C at base pair 72. The original change to get rid of the internal SpeI site was realized to actually change the amino acid in the protein. (From the creators). Please see the Part Design page for a note about the BioBrick sequences on this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
//function/recombination/hin
Parameters
ec_numnone
keggstm:STM2772
proteinHin
swissproP03013