Difference between revisions of "Part:BBa J23103:Experience"
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===Applications of BBa_J23103=== | ===Applications of BBa_J23103=== | ||
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| + | ====Evaluation of Anderson promoter J23103 in ''B. subtilis'' by iGEM-Team LMU-Munich 2012==== | ||
| + | This Anderson promoter was evaluated without fused RFP with the ''lux'' operon as a reporter in ''B. subtilis''. See the new BioBrick [https://parts.igem.org/Part:BBa_K823007 BBa_K823007] without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in ''B. subtilis''. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 10:02, 26 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J23103
Evaluation of Anderson promoter J23103 in B. subtilis by iGEM-Team LMU-Munich 2012
This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823007 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.
User Reviews
UNIQ264b9208fcdc90b1-partinfo-00000000-QINU
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iGEM HKU 2011 |
To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain. The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression. UNIQ264b9208fcdc90b1-partinfo-00000002-QINU |
