Difference between revisions of "Part:BBa I757014:Design"
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<partinfo>BBa_I757014 short</partinfo> | <partinfo>BBa_I757014 short</partinfo> | ||
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===References=== | ===References=== | ||
+ | ''Not a comprehensive list'' | ||
+ | |||
+ | <biblio> | ||
+ | #Schmidt93 pmid=8433964 | ||
+ | // this looks to be the original reference for the ''Strep''-tag | ||
+ | #Skerra96 pmid=8636976 | ||
+ | // original reference for ''Strep''-tag II | ||
+ | #Voss97 pmid=9415448 | ||
+ | // original reference for streptavidin mutant with enhanced affinity for ''Strep''-tag and ''Strep''-tag II. The variant was later called StrepTactin. | ||
+ | #Skerra99 pmid=10796988 | ||
+ | #Skerra00 pmid=11036648 | ||
+ | // history of ''Strep''-tag system | ||
+ | #Skerra07 pmid=17571060 | ||
+ | // includes brief overview of ''Strep''-tag system | ||
+ | </biblio> |
Latest revision as of 20:30, 13 August 2009
Strep-Tag II affinity tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions
Source
synthetic DNA by GeneArt
References
Not a comprehensive list
<biblio>
- Schmidt93 pmid=8433964
// this looks to be the original reference for the Strep-tag
- Skerra96 pmid=8636976
// original reference for Strep-tag II
- Voss97 pmid=9415448
// original reference for streptavidin mutant with enhanced affinity for Strep-tag and Strep-tag II. The variant was later called StrepTactin.
- Skerra99 pmid=10796988
- Skerra00 pmid=11036648
// history of Strep-tag system
- Skerra07 pmid=17571060
// includes brief overview of Strep-tag system </biblio>