Device

Part:BBa_I724000

Designed by: Hanmo Li   Group: iGEM07_McGill   (2007-10-26)


Autoinducer inactivation enzyme regulated by LuxR/HSL promoter

We made this construct with LuxR/HSL promoter R0062, B0034 RBS, and the C0060 aiiA gene in order to give us more control with our repressilator system. The aiiA gene will be turned on by the LuxR/HSL promoter in the presence of the LuxR activator protein complexed with the autoinducer 3-oxo-hexanoyl-HSL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/cellsignalling
//function/degradation/ahl
Parameters
None