Part:BBa_I719005

T7 Promoter

Constitutive promoter derived from the T7 bacteriophage. Allows high expression of proteins only when the T7 polymerase is present. This part is identical to the part BBa_R0085 which currently hasn't been built.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization in vitro

This part has been characterized for temperature sensitivity in the Cell-Free Chassis by iGEM Imperial 2007. For more detail, please check the testing [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Protocols/Prot1.9 protocols]and [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Results/Res1.9 results].

Parameter	Value and Description
Optimum temperature	The T7 promoter has a highest output at 25°C, with a one-fold increase in GFP molecules synthesized compared to 37°C. The T7 promoter also has a minimal amount of output at 4°C.
Expression Life-span	The rate of GFP synthesis by the T7 promoter reaches a peak at around 30-60 minutes.
Peak Expression	The production of GFP decreases to minimal levels after 2 hours and tends towards nil after 4 hours.