RBS

Part:BBa_I20299

Designed by: Francois St-Pierre   Group: Endy Lab   (2010-06-21)


RBS from T7 gene 4, optimal aligned spacing

RBS derived from phage T7 gene 4. Similar to I20298, but with the last (3') nucleotides removed. This produces an optimal aligned spacing of 5 nucleotides between the Shine-Dalgarno (SD) sequence and the ATG of the following CDS, when the RBS is placed immediately before the ATG i.e. without Biobrick "scar". For information on "optimal aligned spacing", see Chen et al., (1994) NAR, 22(23):4953-7 [PMID 7528374]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None