Signalling

Part:BBa_I13015

Designed by: Debra, Fred, Jay, Madeleine, Cell-Cell Signaling SMUG   Group: Antiquity   (2004-07-08)
Revision as of 16:42, 17 October 2018 by Felipebuson (Talk | contribs)


3OC6HSL Sender controlled by pBad

The pBad promoter controlling a LuxI sender device. The protein is an untagged LuxI enzyme which produces an AHL (3OC6HSL).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1873
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Activity compared to synthetic 3OC6 HSL</h> Team USP-Brazil characterized this part by using it in a quorum sensing system along with LuxR and promoter pLux (see BBa_K2771030), in an assay comparing pBad at full activity (2.5mM arabinose) and a quantity known to activate well pLux (10-4 mM HSL

[edit]
Categories
//classic/signalling/sender
Parameters
device_typeSender