Difference between revisions of "Part:BBa E1010:Design"

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__NOTOC__
 
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<partinfo>BBa_E1010 short</partinfo>
 
<partinfo>BBa_E1010 short</partinfo>
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===Source===
 
===Source===
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<biblio>
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Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
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#Zhang pmid=12461557
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#Campbell pmid=12060735
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</biblio>
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<a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
  
 
===References===
 
===References===
 
Zhang J, Campbell RE, Ting AY, Tsien RY (2002) "Creating New Fluorescent Probes for Cell Biology,"''Nat Rev Mol Cell Biol.''  '''3'''(12), 906-18.  This key reference gives a summary of the details of engineering the palette of fluorescent proteins. (SCM)
 
Zhang J, Campbell RE, Ting AY, Tsien RY (2002) "Creating New Fluorescent Probes for Cell Biology,"''Nat Rev Mol Cell Biol.''  '''3'''(12), 906-18.  This key reference gives a summary of the details of engineering the palette of fluorescent proteins. (SCM)

Revision as of 20:57, 16 July 2009

**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TAATAA double stop codon added (DE).

Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.


Source

<biblio>

  1. Zhang pmid=12461557
  2. Campbell pmid=12060735

</biblio> <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>

References

Zhang J, Campbell RE, Ting AY, Tsien RY (2002) "Creating New Fluorescent Probes for Cell Biology,"Nat Rev Mol Cell Biol. 3(12), 906-18. This key reference gives a summary of the details of engineering the palette of fluorescent proteins. (SCM)