DNA
SapI

Part:BBa_B0102

Designed by: Heather Keller   Group: T7.2   (2007-03-05)


SapI restriction enzyme site (offset cutter)

SapI is an offsite cutter restriction site. The recognition sequence for this enzyme is the 8 bp sequence GCTCTTCN (on the 5' strand). Cleavage at this site results in a 3 bp, 3' overhang (right side, bottom strand) directly downstream of this sequence. This specific part has a T in the 8th position.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1


[edit]
Categories
//DNA/restrictionsite
Parameters
None