Part:BBa_M50007

U1 Thermosensitive 5'-UTR (includes RBS)

Organism: Escherichia coli

It utilizes the distinct thermosensor U1, with different expression patterns at various temperature levels.

At elevated temperatures, the U1 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. We picked U1 because of its unique trait that allows for the detection of increasing gene expression levels ranging from 22˚C to 37˚C. We did not make any modifications to the thermosensor genetic sequences given by Neupert et. al.

Our plasmid includes a standard selectable marker chloramphenicol, pP-T5 promoter, the U1 RBS, mTurquoise FP, pT-T7 terminator, and a high copy ORI.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 13
  Illegal PstI site found at 19
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 13
  Illegal PstI site found at 19
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 13
  Illegal PstI site found at 19
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 13
  Illegal PstI site found at 19
  
• 1000
  COMPATIBLE WITH RFC[1000]