Coding

Part:BBa_K902019

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-02)

S7 micrococcal nuclease

S7 micrococcal nuclease, also known as MNase is native to Staphylococcus aureus uses this enzyme to destroy extracellular DNA when it infects humans to evade the immune system. S7 has both endo and exonuclease activity, and has a preference for -AT rich regions as opposed to -GC rich regions. Due to the non-specificity and high activity of this enzyme, it digests the DNA into <200 bp fragments. Ideally this enzyme will be present both intracellularly and extracellularly. The intracellular fraction would degrade the intracellular genetic material and the extracellular fraction would degrade any free floating DNA thereby reducing the chances of horizontal gene transfer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None