Composite

Part:BBa_K875007

Designed by: Marija Drikic, Giorgio Gargari   Group: iGEM12_Trieste   (2012-09-23)


T5 LacO-PelB-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT

This construct is designed for the expression of secreted, already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6). The antibody is expressed in a small immuno protein (SIP) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker and constant domain (CH3) of heavy chain of human immunoglobulin A (IgA). It has already been reported that the scFv 54.6 (which makes part of SIP) binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. Alpha isotype CH3 domain homodimerizes confering bivalent binding properties to the antibody. PelB is a leader sequence often used to produce secreted proteins.

The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, PelB-SIP 54.6 antinorovirus, Hystidine tag (6HIS), Terminator (B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 160
    Illegal AgeI site found at 1367
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None