Composite

Part:BBa_K824009

Designed by: Steven Allen   Group: iGEM12_Gaston_Day_School   (2012-10-02)

Arsenic Promoter with GFP Reporter (Arsenic Detector)

We digested the promoter responsive to Arsenic(BBa_J33201) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168


[edit]
Categories
//function/sensor/metal
Parameters
None