RBS

Part:BBa_K812059

Designed by: Hafez El Sayyed   Group: iGEM12_Evry   (2012-09-26)


A GoldenBricked version of week RBS J61117

J61117 is a week-strength RBS belonging to a family of similar ribosome binding sites basic parts identified from a saturation mutagenic library. This part was modified to adjust to Goldenbrick assembly prrotocol

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 11
    Illegal SpeI site found at 31
    Illegal PstI site found at 43
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 31
    Illegal PstI site found at 43
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 11
    Illegal SpeI site found at 31
    Illegal PstI site found at 43
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 11
    Illegal SpeI site found at 31
    Illegal PstI site found at 43
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 37


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