Composite

Part:BBa_K801037

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM12_TU_Munich   (2012-09-21)

Linker-GAL4 DNA binding domain

Composite part of BBa_K365005 and BBa_K801032.

This fusion protein contains a 20 amino acids long linker in the N-terminus and the GAL4 DNA binding domain in the C-terminus ((N'-20aaLinker-GAL4DBD-C')). The protein linker improves the folding of protein domains fused to the N-terminus of this part because folding is spatially and temporary spaced by the linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 200


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain
//proteindomain/dnabinding
Parameters
None