Coding

Part:BBa_K783054

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)

This is a MoClo converted version of BBa_C0079

This is a MoClo converted version of BBa_C0079, which is the coding region for the lasR protein, which accepts chemical signal AI-1 (made by lasI protein).

It has two MoClo fusion sites flanking it: 5' C [part] D 3'

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//function/cellsignalling
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligandsPAI
proteinlasR-LVA
swissproP25084
tagLVA