Coding
Part:BBa_K783054
Designed by: Traci Haddock Group: iGEM12_BostonU (2012-10-02)
This is a MoClo converted version of BBa_C0079
This is a MoClo converted version of BBa_C0079, which is the coding region for the lasR protein, which accepts chemical signal AI-1 (made by lasI protein).
It has two MoClo fusion sites flanking it: 5' C [part] D 3'
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
Illegal AgeI site found at 519 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
//cds/transcriptionalregulator/activator
//function/cellsignalling
//function/regulation/transcriptional
//cds/transcriptionalregulator/activator
//function/cellsignalling
//function/regulation/transcriptional
direction | Forward |
function | Rep |
ligands | PAI |
protein | lasR-LVA |
swisspro | P25084 |
tag | LVA |