Composite

Part:BBa_K777114

Designed by: Team Goettingen   Group: iGEM12_Goettingen   (2012-09-21)

motA under the control of constitutive promoter J23100

The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation.
Here we used 3 different constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive motA expression affect the motility of E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 45
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 813


[edit]
Categories
//cds/membrane
//chassis/prokaryote/ecoli
//function/motility
//promoter/anderson
Parameters
chassisE. coli
functionMotility
positive_regulatorsConstitutive promoter
proteinmotA