Part:BBa_K777110

fliC under the control of constitutive promoter J23100

The fliC gene codes for flagellin. This protein is the subunit that forms the flagellar filament of E. coli.

Here we used 3 different constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive fliC expression affect the motility or flagellum size of E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1265
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 342
  Illegal AgeI site found at 750
  
• 1000
  COMPATIBLE WITH RFC[1000]