RNA

Part:BBa_K635000

Designed by: Arjun Athreya   Group: iGEM11_Virginia   (2011-09-27)


siRNA gene silencer for use in yeast

This siRNA fragment is designed for modular inhibition of translation for various synthetic biology systems in yeast. The siRNA is reverse complementary to the biobrick kozak sequence BBa_J63003 and a randomly generated buffer sequence that should precede it GGAGTTGTGGGCACCTGCTA. The idea is similar to the RSID system developed by the Stanford 2010 iGEM team for siRNA control in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None