Translational_Unit

Part:BBa_K622006

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-02)

ChitinaseA1


This part codes ChiA1 and its RBS. Its start codon is not AUG but GUG.

ChiA gene we used is derived from Streptomyces avermitilis. S. avermitilis is a kind of prokaryotic bacteria and they produce chitinase. [http://www.ncbi.nlm.nih.gov/pubmed/12692562],[http://www.ncbi.nlm.nih.gov/pubmed/11572948]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
    Illegal NgoMIV site found at 365
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1615
    Illegal NgoMIV site found at 1634
    Illegal AgeI site found at 212
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
None