RNA

Part:BBa_K587012

Designed by: David Ignacio Maycotte Cervantes   Group: iGEM11_ITESM_Mexico   (2011-10-09)


iTast -> Antikey sequence for high concentration mechanism

This sequence forms part of the group of pieces directed to the expression of a concentration mechanism developed by our team ITESM_MEXICO 2011. The inactivation of gene expression is achieved by transcribing a non-coding RNA (itastRNA) produced by a third promoter (following activation of pBADwk in the presence of arabinose). This mRNA targets the tastRNA specifically. The itastRNA is designed to lock again the complex crstRNA-RBS due to hybridization with tastRNA; inhibiting the expression of the reporter protein at low concentration. It allows only the expression of the high concentration mechanism.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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