RNA

Part:BBa_K576004

Designed by: George Zarubin   Group: iGEM11_Waterloo   (2011-06-20)


Right Part of Self-Excising Ribozyme

Modified 3' "half" of intron found in ORF #142 in Twort phage of Staphylococcus aureus in RFC53 MetaPart format. When complemented with 5' "half" BBa_K576003 in complience with BBFRFC53 assembly standard, the RNA-level excision should leave GGU linker between the whatever comes before K576003 and after K576004. K576003 and K576004 and everything in between them would be removed and possibly circularized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3


[edit]
Categories
Parameters
None