Composite

Part:BBa_K575036

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

This construct is designed to report the presence of PAI2, part of the Pseudomonas quorum sensing hierarchy. When induced with PAI-2 (C4-HSL) at varying concentrations, this construct fluoresced more than the control. Which allows us to use this part as a biosensor for the detection of Pseudomonas Aeruginosa. Rhlp-34-gfp cp-30-rhlr.jpg


RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP + Constitutive Promoter + RBS (B0030) + RhlR

Continuous expression of RhlR (with RBS B0030), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 814
    Illegal NheI site found at 837
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1111
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1586
    Illegal BsaI.rc site found at 723


[edit]
Categories
Parameters
None