Conjugation

Part:BBa_K543016

Designed by: Naoto Shimada   Group: iGEM11_Tokyo_Metropolitan   (2011-10-03)


Bacteriophage Lysis Cassette S105, R, and Rz under Tet promoter (conjugative)

Bacteriophage Lysis Cassette S105（Holin), R(Endolysin), and Rz under pTet.

Using this device with tetR generator, cell lysis is repressed. But adding aTc, cell lysis is induced.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 278
    Illegal NheI site found at 324
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None