Part:BBa_K5384012

biology Natural transcriptional termination and polyadenylation signals from the AOX1 gene, including the last 27 codons of the AOX1 open reading frame, allow for efficient 3' mRNA processing, including polyadenylation, to improve mRNA stability in Pichia pastoris.

AOX terminator Natural transcriptional termination and polyadenylation signals from the AOX1 gene, including the last 27 codons of the AOX1 open reading frame, allow for efficient 3' mRNA processing, including polyadenylation, to improve mRNA stability in Pichia pastoris.Controlling gene expression is often the foremost goal of most biological endeavors like the production of industrial enzymes and expression of heterologous metabolic pathway genes. The components of the entire "expression cassette" exert control on net protein output. This control is primarily achieved through altering the promoter driving expression and by changing the copy number of the gene. However, there are only a few recent studies on terminators. Terminators are essential components in expression cassettes that influence the 3' end processing of mRNA, mRNA stability, and translational efficiency, which can modulate protein producti

Secondary Structure

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Measurement

• How these parts were measured

AOX terminator

Natural transcriptional termination and polyadenylation signals from the AOX1 gene, including the last 27 codons of the AOX1 open reading frame, allow for efficient 3' mRNA processing, including polyadenylation, to improve mRNA stability in Pichia pastoris.[1] Controlling gene expression is often the foremost goal of most biological endeavors like the production of industrial enzymes and expression of heterologous metabolic pathway genes. The components of the entire "expression cassette" exert control on net protein output. This control is primarily achieved through altering the promoter driving expression and by changing the copy number of the gene. However, there are only a few recent studies on terminators. Terminators are essential components in expression cassettes that influence the 3' end processing of mRNA, mRNA stability, and translational efficiency, which can modulate protein production.

Application

A terminator is a DNA sequence that gives the RNA polymerase transcription termination signal. In an operon, there is at least one terminator after the last gene of the structural gene population. The terminator on the plasmid gives the RNA polymerase transcription termination signal, helping to stop the expression of fragments that do not have the gene of interest.

References

1.ROBAINAS-DEL-PINO YANELIS, VIADER-SALVADÓ JOSÉ MARÍA, HERRERA-ESTALA ANA LUCÍA, et al. Functional characterization of the Komagataella phaffii1033 gene promoter and transcriptional terminator[J]. World Journal of Microbiology&Biotechnology,2023,39(9). DOI:10.1007/s11274-023-03682-5. 2.RAMAKRISHNAN, KAMATCHI, PRATTIPATI, MAHESH, SAMUEL, PREMSINGH, et al. Transcriptional control of gene expression in Pichia pastoris by manipulation of terminators[J]. Applied Microbiology and Biotechnology,2020,104(18):7841-7851. DOI:10.1007/s00253-020-10785-8.