Terminator

Part:BBa_K5384011

Designed by: Si Cheng   Group: iGEM24_HBUT-Wuhan   (2024-09-29)

AOX 1 promoter

The AOX 1 terminator is a sequence used to terminate transcription, which helps to ensure that the mRNA of an expressed gene is correctly sheared and stabilized. In the yeast expression system, the AOX 1 terminator plays an important role in ensuring that the expression of exogenous genes can be effectively regulated.


Usage and Biology

In Pichia pastoris, Thomas et al. compared the expression of Pichia pastoris novel termination sequences with the commonly used AOX 1 termination sequences, and finally screened 15 Pichia pastoris termination sequences and 5 Saccharomyces cerevisiae termination sequences, and finally found that none of the EGFP (Enhanced Green Fluorescent Protein) expression was as high as the AOX 1 terminator expression level.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]TANG Xiaoyan. Screening and application evaluation of highly efficient transcription termination sequences in Bichiria yeast[D]. Guangdong:South China University of Technology,2019.

[edit]
Categories
//function/biosynthesis
Parameters
biologyNatural transcriptional termination and polyadenylation signals from the AOX1 gene, including the last 27 codons of the AOX1 open reading frame, allow for efficient .