RBS

Part:BBa_K5282010

Designed by: Chengyao Yin   Group: iGEM24_HS-Beta   (2024-10-01)


TfCa CDS

The gram-positive thermophilic actinomycete Thermobifida fusca KW3 secretes a highly hydrophobic carboxylesterase (TfCa) that is able to hydrolyze poly(ethylene terephthalate). The gene is loaded between T7 promoter and T7 terminator, and there is lac operator and ribosome binding site (RBS) between T7 promoter and coding sequence, and the expression of TfCa can be induced by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
    Illegal NotI site found at 1498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal BamHI site found at 308
    Illegal XhoI site found at 1507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
    Illegal NgoMIV site found at 826
    Illegal NgoMIV site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1327
    Illegal SapI.rc site found at 1315


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