Coding

Part:BBa_K525009

Designed by: Manuel Wittchen   Group: iGEM11_Bielefeld-Germany   (2011-10-25)


Fusion Protein of S-Layer SgsE and LigA with His-Tag

Fusion Protein of S-Layer SgsE and LigA with His-Tag

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1022
    Illegal BglII site found at 3819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1657
    Illegal SapI.rc site found at 4033


[edit]
Categories
//cds/enzyme/ligase
//chassis/prokaryote/ecoli
//proteindomain/internal
//rnap/bacteriophage/t7
Parameters
biologyS-Layer
chassisE. coli
controlPT7
originGeobacillus stearothermophilus, Escherichia coli
tagHis-tag