RBS

Part:BBa_K5226024

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-08-18)

RBS for Vib-gcvT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens.

Usage and Biology

This part is a native ribosomal binding site of Vib-gcvT, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts to facilitate the expression of aminomethyltransferase.


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Categories
Parameters
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