Part:BBa_K5223006

HCT-M (Hydroxycinnamoyl transferase)

Hydroxycinnamoyl transferase (HCT) is a crucial member of the plant acyltransferase family. HCT has been extensively applied in developing bioenergy, crop improvement, and anti-inflammatory drug production, playing a significant role in the synthesis and post-modification of plant secondary metabolites. Studies have shown that an HCT-M enzyme from red clover exhibits a high affinity for malic acid, and introducing this gene into Nicotiana benthamiana provides the essential activity for the synthesis of caffeoyl malic acid. We optimized the codons for E. coli to enhance the gene's expression efficiency in this bacterial system.

Biology and Usage

HCT-M
Function	Synthesize caffeoyl shikimic acid
Use in	Nicotiana benthamiana
Backbone	pZYB11-pEAQ-HT-DEST1
Derived from	Trifolium pratense

Reference

[1] Michael L. Sullivan, Robert Zarnowski, Red Clover HCT2, a Hydroxycinnamoyl-Coenzyme A: Malate Hydroxycinnamoyl Transferase, Plays a Crucial Role in Biosynthesis of Phaselic Acid and Other Hydroxycinnamoyl-Malate Esters in Vivo, Plant Physiology, Volume 155, Issue 3, March 2011, Pages 1060–1067, https://doi.org/10.1104/pp.110.166793

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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