Part:BBa_K5223004

GjALDH2C3_opt (aldehyde dehydrogenase)

Aldehyde dehydrogenase (ALDH2C3) was used in this project, which will catalyze the conversion of crocin dialdehyde to crocin [1]. Gardenia is used in traditional Chinese medicine and is of interest as a rich source of crocin [3]. The ALDH family is widely distributed in plants and can oxidize aldehyde oxides to the corresponding carboxylic acid compounds. However, ALDH shows strong specificity in catalyzing crocin dialdehyde [2]; as an essential step in the synthesis pathway, the auxiliary role of ALDH is vital, and it can significantly enhance crocin accumulation. Therefore, we hope to introduce this gene to improve the accumulation of crocin while perfecting the crocin synthesis pathway. ALDH2C3 was detected to be highly expressed in the red fruits of Gardenia jasminoides. In a study, the authors introduced the aldehyde dehydrogenase gene GjALDH2C3 and other genes related to carotenoid synthesis. They produced crocin at a maximum level of 78,362 ng/g FW in Hon's tobacco [2].

Biology and Usage

aldehyde dehydrogenase
Function	Crocin synthesis
Use in	Nicotiana benthamiana
Backbone	pZYB11-pEAQ-HT-DEST1
Derived from	Gardenia jasminoides

Reference

[1] Ji A, Jia J, Xu Z, Li Y, Bi W, Ren F, He C, Liu J, Hu K, Song J. Transcriptome-Guided Mining of Genes Involved in Crocin Biosynthesis. Front Plant Sci. 2017 Apr 11;8:518. doi: 10.3389/fpls.2017.00518. PMID: 28443112; PMCID: PMC5387100.
[2] Xie L, Luo Z, Jia X, Mo C, Huang X, Suo Y, Cui S, Zang Y, Liao J, Ma X. Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana. International Journal of Molecular Sciences. 2023; 24(18):14139. https://doi.org/10.3390/ijms241814139
[3] Frusciante S, Diretto G, Bruno M, Ferrante P, Pietrella M, Prado-Cabrero A, Rubio-Moraga A, Beyer P, Gomez-Gomez L, Al-Babili S, Giuliano G. Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12246-51. doi: 10.1073/pnas.1404629111. Epub 2014 Aug 5. PMID: 25097262; PMCID: PMC4143034.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]