Part:BBa_K5223002

GjUGT74F8_opt (UDP-glucosyltransferase)

GjUGT74F8 is a UDP-glucosyltransferase derived from Gardenia jasminoides, which can catalyze crocetin to produce crocin Ⅲ and crocin Ⅴ, and catalyze crocin Ⅳ to produce crocin Ⅱ. In engineering E. coli, the conversion rate of GjUGT74F8 to 50 mg/L crocetin to crocin III and crocin V was 66.1%, and the conversion rate of GjUGT74F8 to 50 mg/L crocetin to 5 crocin was 59.6%. The catalytic activity of microbial UGTs(UDP-glucosyltransferase) is much higher than reported, and it is a highly efficient crocin synthetase. Studies have shown that the transfer of this gene into the Nicotiana benthamiana can play the necessary role in the synthesis of crocin.

Biology and Usage

UDP-glucosyltransferase
Function	Crocin synthesis
Use in	Nicotiana benthamiana
Backbone	pZYB11-pEAQ-HT-DEST1
Derived from	Gardenia jasminoides

Reference

[1] Pu X, He C, Yang Y, Wang W, Hu K, Xu Z, Song J. In Vivo Production of Five Crocins in the Engineered Escherichia coli. ACS Synth Biol. 2020 May 15;9(5):1160-1168.
[2] Xie L, Luo Z, Jia X, Mo C, Huang X, Suo Y, Cui S, Zang Y, Liao J, Ma X. Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana. Int J Mol Sci. 2023 Sep 15;24(18):14139.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]