Part:BBa_K5169012
cgrA terminator
Enhance the response of Tet on system to tetracycline and its derivatives, and improve the induction efficiency of the system.
Usage and Biology
The TcgrA terminator is a regulatory sequence derived from the Aspergillus fumigatus cgrA gene, which plays a critical role in ribosome assembly. This terminator sequence functions as a signal that halts transcription by directing RNA polymerase to stop synthesizing RNA once the gene has been transcribed, ensuring proper gene expression regulation.
In genetic engineering, terminators like TcgrA are important tools used to fine-tune gene expression. By signaling the end of transcription, the TcgrA terminator ensures that only the intended portion of the RNA is transcribed, preventing unnecessary or erroneous transcription beyond the target gene. This precise control is essential for maintaining gene function, especially in modified or engineered organisms.
In the context of synthetic biology and molecular biology research, the TcgrA terminator can be incorporated into genetic constructs to ensure stable and efficient transcription termination, which is key to achieving desired levels of gene expression. This makes it a valuable component for designing experiments that require fine control over gene regulation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 153
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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