Part:BBa_K5073039
CD28 TM: Transmembrane region of T-cell-surface glycoprotein CD28
CD28TM is an important synthetic biology component widely used as a co-stimulatory molecule in the chimeric antigen receptor (CAR) system. It is mainly expressed on the surface of T cells and is able to bind to CD80 and CD86 on antigen-presenting cells (APCs), providing the necessary second signals for T cell activation, enhancing T cell proliferation, survival, and cytokine (e.g., IL-2) secretion, and thus improving the function and persistence of CAR-T cells. In CAR design, CD28TM is usually combined with an antigen recognition region (scFv) to form a complete CAR, enabling CAR-T cells to effectively recognize and attack tumor cells, significantly enhancing their anti-tumor activity.CD28TM can be used in a variety of synthetic biology projects, including the development of cancer immunotherapies, vaccine design and basic research. For use, researchers need to clone CD28TM into a suitable plasmid backbone, ensuring that the RBS (ribosome binding site), promoter and terminator are included for proper expression in host cells, and validate its expression by methods such as flow cytometry or Western blot. In functional assays, the effects of CD28TM on T cell proliferation, cytokine production and cytotoxicity are assessed by in vitro stimulation (e.g. co-culture with tumor cells). In addition, CD28TM can be combined with other regulatory elements to dynamically regulate T cell activity and optimize their function. Thus, CD28TM is a core component in the CAR system, and researchers can effectively utilize its advantages to advance the progress of immunotherapy.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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