RNA

Part:BBa_K5047000

Designed by: Clement Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-09-22)


shRNAv1 : DNA sequence encodes a shRNA that does not bind to any genes. It serves as a control.

Long oligo corresponds to shRNA_1 (control) with homology arms for promoter and terminator (to be used as template for amplification with VV_040 and 041).

The aim is to use this shRNA as the control for the rest of our experiments. This shRNA comes from Estonia TUIT Team.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None