Part:BBa_K5038011
LIRA_H210_2/20
Usage and Biology
LIRA_H210_2/20 is a LIRA system that can specifically detect hsa-miR-210-3p and has the same detection principle as LIRA_H01. There is a complementary sequence of hsa-miR-210-3p on the loop of the hairpin structure, and the RBS and initiation codon AUG will be exposed when the complementary sequence binds to the target RNA, thereby activating translation.
"2/20" indicates that this LIRA system has a stem-loop ratio of 2/20 when bound to the corresponding input; that is, in the input sequence, 2 bases are bound to the stem of the LIRA hairpin and 20 bases are bound to the loop.
(Figure 1. Schematic of LIRA_H210_2/20)
Blue: miRNA binding site, miR-210-3p binds to this area
Purple: RBS
Yellow: AUG, initiation codon
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 26
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
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