Part:BBa_K5038010
LIRA_H142_11/12
Usage and Biology
LIRA_H142_11/12 is a LIRA system that can specifically detect hsa-miR-142-3p and has the same detection principle as LIRA_H01. There is a complementary sequence of hsa-miR-142-3p on the loop of the hairpin structure, and the RBS and initiation codon AUG will be exposed when the complementary sequence binds to the target RNA, thereby activating translation.
"11/12" indicates that this LIRA system has a stem-loop ratio of 11/12 when bound to the corresponding input; that is, in the input sequence, 11 bases are bound to the stem of the LIRA hairpin and 12 bases are bound to the loop.
(Figure 1. Schematic of LIRA_H142_11/12)
Blue: miRNA binding site, miR-142-3p binds to this area
Purple: RBS
Yellow: AUG, initiation codon
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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