RNA
Part:BBa_K5038009
Designed by: Qiyu Tan Group: iGEM24_CJUH-JLU-China (2024-09-14)
LIRA_H142_8/15
Usage and Biology
LIRA_H142_8/15 is a LIRA system that can specifically detect hsa-miR-142-3p and has the same detection principle as LIRA_H01. There is a complementary sequence of hsa-miR-142-3p on the loop of the hairpin structure, and the RBS and initiation codon AUG will be exposed when the complementary sequence binds to the target RNA, thereby activating translation.
"8/15" indicates that this LIRA system has a stem-loop ratio of 8/15 when bound to the corresponding input; that is, in the input sequence, 8 bases are bound to the stem of the LIRA hairpin and 15 bases are bound to the loop.
(Figure 1. Schematic of LIRA_H142_8/15)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 13
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |