Part:BBa_K5038005

LIRA_input05

Usage and Biology

LIRA_input05 is the target to be detected by LIRA_H05. It will bind to the loop at the top of LIRA_H05, changing its conformation so that the ribosome can bind to the RBS.

(Figure 1. LIRA_input05 binds to LIRA_H05)

Characterization

In our experiments, we used EGFP as the reporter gene and input05 as the target RNA, and inferred the opening of the hairpin through the fluorescence intensity. Detailed information is provided in BBa_K5038020and BBa_K5038022.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]