Part:BBa_K5038004

LIRA_input01

Usage and Biology

LIRA_input01 is the target to be detected by LIRA_H01. It will bind to the loop at the top of LIRA_H01, changing its conformation so that the ribosome can bind to the RBS.

(Figure 1. LIRA_input01 binds to LIRA_H01)

Characterization

In our experiments, we used EGFP as the reporter gene and input01 as the target RNA, and inferred the opening of the hairpin through the fluorescence intensity. Detailed information is provided in BBa_K5038019and BBa_K5038021.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]