RNA

Part:BBa_K4974000

Designed by: Mustafo Mustafokulov   Group: iGEM23_NYCEmpireState   (2023-10-06)


Thrombin-29 aptamer

The thrombin-29 aptamer was originally characterized by Tasset et al. in 1997. The 29mer specifically targets the heparin-binding site through hydrophobic interactions of the duplex, and is believed to have higher binding affinity than other aptamers.

The thrombin-29 aptamer can be used as a positive control in studies of experimental aptamers. The 2023 NYCEmpireState team incubated thrombin-29 aptamer with thrombin as a positive-control to determine that the team's electrophoretic mobility shift assay (EMSA) was functional.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None